Baby Love Club

Baby Love - Baby Name - Colic Babies - Teething Babies

Newborn Babies - Babies Cry - Baby Needs - Fussy Babies






Little Miss Burnett: A Babylit Flower Primer

RRP $14.99

BabyLit is a fashionable way to introduce your toddler to the world of classic literature. With clever, simple text by Jennifer Adams, paired with stylish design and illustrations by Sugar's Alison Oliver, these books are a must for every savvy parent's nursery library.

About the Author

Jennifer Adams is the author of a dozen books including 'Remarkably Jane: Notable Quotations on Jane Austen' and the popular 'Y Is for Yorick: A Slightly Irreverent ABC Book for Grown-Ups'. Jennifer works as a writer and editor. Alison Oliver runs Sugar design studio. Alison's design portfolio includes everything from logos to packaging and product design for clients such as Chronicle Books, Citibabes, and Aerie, as well as Gibbs Smith.


Sensor Technology For Water Quality Monitoring

RRP $54.95

Two methods for the detection of important human pathogens, Cryptosporidium parvum and Helicobacter pylori, were investigated: a fiber optic biosensor, and real time PCR. The mechanism for specific detection in both methods is recognition of specific DNA sequences in the target organisms. The biosensor that was used, the Analyte 2000, was originally developed for the detection of chemicals. It utilizes a fiber optic wave guide that propagates an evanescent light wave of very specific wavelength. The light excites fluorescent molecules bound to the waveguide, but not in the bulk solution, which theoretically enhances signal while reducing background interference. Attempts to develop this system for the detection of DNA were not successful due to poor detection of the target molecules. An assay analogous to a sandwich immunoassay was designed for use on the Analyte 2000. Specific oligonucleotide probes were designed to bind to the waveguides via biotin-streptavidin interaction, and were used to capture the target DNA. Pure target DNA representing unique genes in the organisms were synthesized by PCR. Detection of captured DNA was then attempted using an oligonucleotide detection probe designed to bind to the target. Two detection systems were employed: an indirect signal amplification system based on biotin-tyramide deposition, or direct detection of fluorescent signal from Cy-5 molecules. In all experiments performed there was very little difference between the signal generated with or without the target molecules. Many experiments were conducted to attempt to identify reasons for the poor signal. Signal was only of any significance when target amplicons were internally labeled with Cy-5 by PCR. Real time PCR as a method to detect the pathogens was also investigated. Though the PCR technique itself is very rapid, DNA extraction and purification requires preparation time. Filtration of up to one liter of well water, followed by concentration and "cleaning" Helicobacter pylori cells by immunomagnetic separation, was used to detect H. pylori seeded in a water source. Following cell lysis, the extracted DNA could be used directly in conventional PCR targeting the 16S rRNA gene to detect less than 265 cells per liter of water. DNA purification was not required for this level of detection. Initial studies to amplify lysed cells by real time PCR indicated that an incorrect product was made. When purified DNA was used for real time PCR, the correct product was produced from DNA representing as few as 100 cells. This publication can be purchased and downloaded via Pay Per View on Water Intelligence Online - click on the Pay Per View icon below



Search

Baby Love Club Articles

Baby Love Baby Name Colic Babies Teething Babies
Newborn Babies Babies Cry Baby Needs Fussy Babies
Baby Chair Baby Slings Baby Monitor Baby Chance

Baby Love Club Books

Baby Love Baby Name Colic Babies Teething Babies
Newborn Babies Babies Cry Baby Needs Fussy Babies
Baby Chair Baby Slings Baby Monitor Baby Chance

Baby Love Club